Transgenic Mouse Facility

The Transgenic Mouse Facility is a Shared Resource of the Duke Cancer Institute. The Facility provides services for the production of designer mutations in mice. We specialize in microinjection of DNA constructs into the pronucleus of fertilized mouse embryos and also provide services for gene targeting experiments using pluripotent mouse embryonic stem cells. The BAC Recombineering Core, a new branch of the Transgenic Facility in 2009, provides services in design and production of DNA constructs using available BAC clones.

Other services related to work with rodents are available through the Department of Lab Animal Resources at Duke. Rodent Breeding Services offers complete colony management in the DLAR Breeding Core. Rodent Genetic Services offers rederivation, cryopreservation of embryos and sperm, in vitro fertilization (IVF), and genotyping. Rodent Technical Services offers a wide variety of technical and surgical services to the Duke Research Community.

Investigators can maximize project efficiency by taking advantage of complete services — to design a gene mutation, generate the construct and screening reagents, target ES cells to produce the genetically altered mice, have the chimeric mice bred and the colony expanded, and preserve the homozygous mice in a frozen state — all by service groups located on Duke’s campus.